Improved radioimaging and tumor localization with monoclonal F(ab')2
Monoclonal anti-tumor antibodies have great promise for radioimmunodetection and localization of tumors. Fab and F(ab')2 fragments, which lack the Fc fragment of antibody (Ab), are cleared more rapidly from the circulation and may have less nonspecific tissue binding than intact Ab. In radioimaging studies using a murine monoclonal antibody to carcinoembryonic antigen in a human colon carcinoma xenografted into hamsters, F(ab')2 fragments were shown superior to Fab fragments and intact antibody for scintiscanning. In double-label experiments with anti-CEA antibody and control monoclonal IgG, F(ab')2 fragments were found to give better and more rapid specific tumor localization than intact antibody or Fab fragments. F(ab')2 fragments offer significant promise for tumor imaging and possibly therapy.
- Research Organization:
- Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, Missouri
- OSTI ID:
- 5764410
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 24:4; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGENS
BODY
CARCINOEMBRYONIC ANTIGEN
CARCINOMAS
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DISEASES
GASTROINTESTINAL TRACT
IMAGES
INTESTINES
LARGE INTESTINE
MONOCLONAL ANTIBODIES
NEOPLASMS
ORGANS
RADIOISOTOPE SCANNING
SCINTISCANNING
USES