Membrane organization of cytochrome c oxidase
The membrane organization of mitochondrial cytochrome c oxidase and the role of intracomplex, intercomplex, rotational and lipid versus protein motion to that organization, has been investigated by amino acid chemical modification and electron paramagnetic resonance. Crosslinking of purified and reconstituted cytochrome c oxidase and mitochondria with biimidates, in the presence and absence of cytochrome c, has been used to investigate oxidase subunit interactions, and whether cytochrome c mobility is required for electron transport activity. The rotational mobility of spin-labeled oxidase in purified form, and incorporated into liposomes, was also studied to gain information on the membrane organization of oxidase. These findings have led to a model for the structure and membrane organization of cytochrome oxidase, and the role of cytochrome c rotational and/or translational motion on the mitochondrial inner membrane surface.
- Research Organization:
- California Univ., Berkeley (USA); California Univ., Berkeley (USA). Lawrence Berkeley Lab.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5761548
- Report Number(s):
- LBL-9888
- Country of Publication:
- United States
- Language:
- English
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