Neuropeptide Y (NPY) and peptide YY (PYY) receptors in rat brain

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Cellular and Molecular Neurobiology; (USA)

DOI:https://doi.org/10.1007/BF00712847· OSTI ID:5761377

Ohkubo, T; Niwa, M; Yamashita, K; Kataoka, Y; Shigematsu, K ^[1]

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1. Nagasaki Univ. School of Medicine, (Japan)

1. Specific binding sites for neuropeptide Y (NPY) and peptide YY (PYY) were investigated in rat brain areas using quantitative receptor autoradiography with {sup 125}I-Bolton-Hunter NPY ({sup 125}I-BH-NPY) and {sup 125}I-PYY, radioligands for PP-fold family peptides receptors. 2. There were no differences between localization of {sup 125}I-BH-NPY and {sup 125}I-PYY binding sites in the rat brain. High densities of the binding sites were present in the anterior olfactory nucleus, lateral septal nucleus, stratum radiatum of the hippocampus, posteromedial cortical amygdaloid nucleus, and area postrema. 3. In cold ligand-saturation experiments done in the presence of increasing concentrations of unlabeled NPY and PYY, {sup 125}I-BH-NPY and {sup 125}I-PYY binding to the stratum radiatum of the hippocampus, layer I of the somatosensory frontoparietal cortex, molecular layer of the cerebellum, and area postrema was single and of a high affinity. There was a significant difference between the affinities of {sup 125}I-BH-NPY (Kd = 0.96 nM) and {sup 125}I-PYY binding (Kd = 0.05 nM) to the molecular layer of the cerebellum. The binding of the two radioligands to the other areas examined had the same affinities. 4. When comparing the potency of unlabeled rat pancreatic polypeptide (rPP), a family peptide of NPY and PYY, to inhibit the binding to the areas examined, rPP displaced {sup 125}I-BH-NPY and {sup 125}I-PYY binding to the area postrema more potently than it did the binding to the stratum radiatum of the hippocampus, layer I of the somatosensory frontoparietal cortex, and molecular layer of the cerebellum. 5. Thus, the quantitative receptor autoradiographic method with {sup 125}I-BH-NPY and {sup 125}I-PYY revealed differences in binding characteristics of specific NPY and PYY binding sites in different areas of the rat brain. The results provide further evidence for the existence of multiple NPY-PYY receptors in the central nervous system.

• OSTI ID: 5761377
• Journal Information: Cellular and Molecular Neurobiology; (USA), Vol. 10:4; ISSN 0272-4340
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
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AFFINITY
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
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IODINE 125
LIGANDS
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NUCLEI
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