Evidence that eukaryotic initiation factor (eIF) 2 is a cap-binding protein that stimulates cap recognition by eIF-4B and eIF-4F
Journal Article
·
· Journal of Biological Chemistry; (USA)
OSTI ID:5761222
- University of Utrecht (Netherlands)
We studied the mRNA-binding properties of eukaryotic initiation factor (eIF) 2. This Met-tRNA-binding factor interacts with the cap structure of reoviral mRNA in an ATP-independent manner. Both the beta- and gamma-subunit of eIF-2 are involved in the UV-induced cross-linking of eIF-2 to the cap. The interaction of eIF-2 with a messenger is sensitive to the cap analogue 7-methyl-guanosine 5'-triphosphate as measured by cross-linking and by mRNA retention on nitrocellulose filters. The cap-binding property of eIF-2 does not conflict with the current mRNA-binding model of initiation factors eIF-4A, -4B, and -4F: cross-linking of eIF-4E and of eIF-4B is stimulated by eIF-2. The eIF-2-mediated increase of eIF-4E interaction results in a decrease of the cross-linking of the beta- and gamma-subunits of eIF-2. The presence of GTP in the cross-linking assay interferes with the interaction of eIF-2 with the cap structure but does not inhibit the eIF-2 stimulated eIF-4E and -4B cross-linking. These observations indicate a role for eIF-2 in the mRNA recognition.
- OSTI ID:
- 5761222
- Journal Information:
- Journal of Biological Chemistry; (USA), Journal Name: Journal of Biological Chemistry; (USA) Vol. 266:11; ISSN JBCHA; ISSN 0021-9258
- Country of Publication:
- United States
- Language:
- English
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· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
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OSTI ID:5019808
Photoaffinity labeling of the cap-binding protein complex with ATP/dATP. Differential labeling of free eukaryotic initiation factor 4A and the eukaryotic initiation factor 4A component of the cap-binding protein complex with (alpha-TSP)ATP/dATP
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Mon Nov 04 23:00:00 EST 1985
· J. Biol. Chem.; (United States)
·
OSTI ID:6086294
Dissociation of 5' proximal helical regions in messenger RNAs by eukaryotic initiation factors 4F, 4A, and 4B
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6079238
Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHEMICAL PROPERTIES
CHEMICAL REACTIONS
CROSS-LINKING
ELECTROMAGNETIC RADIATION
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIATIONS
RADIOINDUCTION
RNA
ULTRAVIOLET RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHEMICAL PROPERTIES
CHEMICAL REACTIONS
CROSS-LINKING
ELECTROMAGNETIC RADIATION
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIATIONS
RADIOINDUCTION
RNA
ULTRAVIOLET RADIATION