A link between double-strand break-related repair and V(D)J recombination: the scid mutation

Journal Article · Wed May 15 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.88.10.4061· OSTI ID:5760361

Hendrickson, E A; Qin, X Q; Bump, E A; Schatz, D G; Oettinger, M; Weaver, D T ^[1]

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1. Dana-Farber Cancer Institute, Boston, MA (USA)

We show here that mammalian site-specific recombination and DNA-repair pathways share a common factor. The effects of DNA-damaging agents on cell lines derived from mice homozygous for the scid (severe combined immune deficiency) mutation were studied. Surprisingly, all scid cell lines exhibited a profound hypersensitivity to DNA-damaging agents that caused double-strand breaks (x-irradiation and bleomycin) but not to other chemicals that caused single-strand breaks or cross-links. Neutral filter elution assays demonstrated that the x-irradiation hypersensitivity could be correlated with a deficiency in repairing double-strand breaks. These data suggest that the scid gene product is involved in two pathways: DNA repair of random double-strand breaks and the site-specific and lymphoid-restricted variable-(diversity)-joining (V(D)J) DNA rearrangement process. We propose that the scid gene product performs a similar function in both pathways and may be a ubiquitous protein.

• OSTI ID: 5760361
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 88:10; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLEOMYCIN
CONNECTIVE TISSUE CELLS
DISEASES
DNA REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GENE MUTATIONS
GENE RECOMBINATION
GENE RECOMBINATION PROTEINS
IMMUNE SYSTEM DISEASES
IONIZING RADIATIONS
MAMMALS
MICE
MUTATIONS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
STRAND BREAKS
VERTEBRATES
X RADIATION