Modification of monoclonal antibodies by polymers possessing chelating properties
This paper describes a basically new approach to obtaining diagnostic antibodies, consisting of a one-point modification of the antibody, without loss of its activity, by a high-molecular-weight synthetic polymer with the ability of effectively chelating ions of heavy metals. As a result of this approach, preparations of active antibodies containing some tens of atoms of the metal per protein molecule can be obtained. The concentration of radioactive metal (/sup 111/In) was determined with a gamma-counter and the Mn and Cd concentrations by spectroscopy. Gel-filtration of polymer-modified antibodies after binding of /sup 111/InCl/sub 3/ is shown. Also, solid-phase radioimmunoassay of antibodies and Fab fragments, native and modified by chelating polymers, is presented.
- Research Organization:
- All-Union Cardiologic Research Center, Moscow, USSR
- OSTI ID:
- 5757973
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 102:7; Other Information: Translated from Byulleten' Ehksperimental'noj Biologii i Meditsiny; 102: No. 7, 63-65(Jul 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
CHELATING AGENTS
BIOLOGICAL EFFECTS
MONOCLONAL ANTIBODIES
LABELLING
MODIFICATIONS
RADIOIMMUNOASSAY
ORGANIC POLYMERS
BIOCHEMISTRY
CADMIUM
CHELATES
FILTRATION
GAMMA CAMERAS
GAMMA SPECTROSCOPY
INDIUM 111
IODINE 121
IODINE 123
LABELLED COMPOUNDS
MANGANESE
MYOCARDIAL INFARCTION
NMR SPECTRA
RADIOACTIVITY
TECHNETIUM 99
TOMOGRAPHY
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CAMERAS
CARDIOVASCULAR DISEASES
CHEMISTRY
COMPLEXES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
HOURS LIVING RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYMERS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
TRANSITION ELEMENTS
YEARS LIVING RADIOISOTOPES
550901* - Pathology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics