Isolation and characterization of Clostridium acetobutylicum mutants with enhanced amylolytic activity
- Univ. of Illinois, Urbana (United States)
Several schemes have been proposed for the fermentative production of butanol from various low-cost substrates. One of these economically viable approaches depends on use of a stable, high-yielding strain of Clostridium acetobutylicum, low-cost corn substrate and an increased market for butanol. Results from various laboratories suggested that amylolytic enzyme biosynthesis in C. acetobutylicum is subject to catabolite repression by glucose and induction by starch. In this study Clostridium acetobutylicum mutants BA 101 (hyperamylolytic) and BA 105 (catabolite derepressed) were isolated by using N-methyl-N{prime}-nitro-N-nitrosoguanidine together with selective enrichment on the glucose analog 2-deoxyglucose. Amylolytic enzyme production by C. acetobutylicum BA 101 was 1.8- and 2.5-fold higher than that of the ATCC 824 strain grown in starch and glucose, respectively. C. acetobutylicum BA 105 produced 6.5-fold more amylolytic activity on glucose relative to that of the wild-type strain. The addition of glucose at time zero to starch-based P2 medium reduced the total amylolytic activities of C. acetobutylicum BA 101 and BA 105 and 82 and 25%, respectively, as compared with the activities of the same strains grown on starch alone. Localization studies demonstrated that the amylolytic activities of C. acetobutylicum BA 101 and BA 105 were primarily extracellular on all carbohydrates tested.
- OSTI ID:
- 5754985
- Journal Information:
- Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 57:9; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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090900 -- Biomass Fuels-- Processing-- (1990-)
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALDEHYDES
AMYLASE
BACTERIA
BIOSYNTHESIS
BUTANOLS
CARBOHYDRATES
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
ENZYME ACTIVITY
ENZYMES
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROXY COMPOUNDS
METHANOGENIC BACTERIA
MICROORGANISMS
MONOSACCHARIDES
MUTANTS
NITROSO COMPOUNDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
REAGENTS
SACCHARIDES
STARCH
SYNTHESIS