Facilitation of syngeneic stem cell engraftment by anti-class I monoclonal antibody pretreatment of unirradiated recipients

Journal Article · Thu Oct 01 04:00:00 EDT 1987 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198710000-00006· OSTI ID:5753516

Voralia, M; Semeluk, A; Wegmann, T G

We have established a murine model of syngeneic bone marrow transplantation based on the use of monoclonal antibody as the sole conditioning regimen in unirradiated recipients. Administration of a single injection of monoclonal antibody directed against major histocompatibility complex-encoded class I determinants facilitated permanent hemopoietic stem cell engraftment without any apparent side-effects. Whereas untreated hosts exhibited a maximal chimerism of 15% at donor cell doses of up to 12 X 10(7) bone marrow cells, pretreatment by 2 mg of anti-class I antibody one week prior to transplantation of 3 X 10(7) syngeneic bone marrow cells resulted in a mean donor representation of about 80%. The antibody can be given up to four weeks prior to transplantation, and the degree of donor engraftment observed is a function of the dose of antibody administered. The fact that specific antibody enhanced engraftment in two strain combinations indicates that antibody is the active agent in facilitating engraftment and that facilitation is not strain-restricted. Anti-class I antibodies of the IgG2a, but not IgG1, isotype are effective in promoting engraftment. Although the isotype requirement suggests a role for antibody-mediated cytotoxicity in promoting stem cell engraftment, the extensive time-frame of facilitation suggests that other effects of the antibody may also be involved. The model of syngeneic bone marrow transplantation we describe here will be useful in studying the mechanisms regulating stem cell engraftment and may have potential clinical application as an approach to autologous marrow transplantation.

• Research Organization: Univ. of Alberta, Edmonton
• OSTI ID: 5753516
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 44:4; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BONE MARROW CELLS
CHIMERAS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
FUNCTIONS
IMMUNE REACTIONS
MAMMALS
MICE
MONOCLONAL ANTIBODIES
MOSAICISM
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
STEM CELLS
TRANSPLANTS
VERTEBRATES