Laser irradiation of centrosomes in newt eosinophils: evidence of centriole role in motility

Journal Article · Fri Jun 01 04:00:00 EDT 1984 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.98.6.1999· OSTI ID:5752355

Koonce, M P; Cloney, R A; Berns, M W

Newt eosinophils are motile granulated leukocytes that uniquely display a highly visible centrosomal area. Electron microscope and tubulin antibody fluorescence confirms the presence of centrioles, pericentriolar material, and radiating microtubules within this visible area. Actin antibodies intensely stain the advancing cell edges and tail but only weakly stain pseudopods being withdrawn into the cell. Randomly activated eosinophils follow a roughly consistent direction with an average rate of 22.5 ..mu..m/min. The position of the centrosome is always located between the trailing cell nucleus and advancing cell edge. If the cell extends more than one pseudopod, the one closest to or containing the centrosome is always the one in which motility continues. Laser irradiation of the visible centrosomal area resulted in rapid cell rounding. After several minutes following irradiation, most cells flattened and movement continued. However, postirradiation motility was uncoordinated and directionless, and the rate decreased to an average of 14.5 ..mu..m/min. Electron microscopy and tubulin immunofluorescence indicated that an initial disorganization of microtubules resulted from the laser microirradiations. After several minutes, organized microtubules reappeared, but the centrioles appeared increasingly damaged. The irregularities in motility due to irradiation are probably related to the damaged centrioles. The results presented in this paper suggest that the centrosome is an important structure in controlling the rate and direction of newt eosinophil motility.

• Research Organization: Univ. of California, Irvine
• OSTI ID: 5752355
• Journal Information: J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 98:6; ISSN JCLBA
• Country of Publication: United States
• Language: English

Related Subjects

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560400 -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTIN
AMPHIBIANS
ANIMALS
ANTIBODIES
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CYTOLOGICAL TECHNIQUES
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
FLUORESCENCE
LASER RADIATION
LEUKOCYTES
LUMINESCENCE
MATERIALS
MICROSCOPY
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
SALAMANDERS
STAINS
VERTEBRATES