Application of the Hydra attenuata assay for identifying developmental hazards among natural waters and wastewaters
- Haskell Laboratory for Toxicology and Industrial Medicine, E. I. du Pont de Nemours and Company, Newark, Deleware (United States)
This study concerns application of the Hydra attenuata assay to detect the developmental toxicity potential of various aqueous samples. First, the assay was modified for testing aqueous samples because water quality has a major impact on aquatic toxicity testing and the results thus obtained. Ranges of sample pH, salinity (conductivity), and hardness were examined for their adverse effects upon the hydra. Adult hydra were unaffected morphologically by pH 5.5-9.5, and the artificial embryo ( embryo') developed normally in a pH range of 6.25 to 8.25. For water hardness, the minimal affective concentration was 1000 mg/liter (as CaCO3) in adults and 625 mg/liter in the embryos; the NOAELs for these were 750 mg/liter in the adult and 250 mg/liter CaCO3 in the embryo. Salinity in excess of 5 ppt was lethal to adults and embryos, indicating the assay may not be applicable to marine or highly saline samples. Finally, grab samples were tested from rivers in Maryland, Pennsylvania, New Jersey, and Delaware, some of which are impacted by industrial and agricultural activities, as well as several samples of industrial wastewaters from one major facility. The assay functioned normally with these diverse samples and yielded results that can be used in assessing the potential developmental hazard of these materials.
- OSTI ID:
- 5745678
- Journal Information:
- Ecotoxicology and Environmental Safety; (United States), Journal Name: Ecotoxicology and Environmental Safety; (United States) Vol. 22:3; ISSN EESAD; ISSN 0147-6513
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOLOGICAL INDICATORS
CNIDARIA
COELENTERATA
DELAWARE
DEVELOPED COUNTRIES
HYDRA
HYDROGEN COMPOUNDS
INVERTEBRATES
LIQUID WASTES
MARYLAND
MONITORING
NEW JERSEY
NORTH AMERICA
OXYGEN COMPOUNDS
PENNSYLVANIA
PH VALUE
POLLUTION
SALINITY
SEAWATER
SENSITIVITY
USA
WASTE WATER
WASTES
WATER
WATER POLLUTION