Use of denatured radioalbumin for determination of trypsin and chymotrypsin inhibitors in different plant seeds
Journal Article
·
· Journal of Agricultural and Food Chemistry; (United States)
- Univ. of Veterinary Medicine, Kosice (Czechoslovakia)
The procedure for determination of trypsin and chymotrypsin inhibitors with urea-denatured albumin labeled by {sup 125}I is described. The content of both types of inhibitory activity has been determined in crude extracts of soybean, bean, lentil, pea, horse bean, maize, and 20 pea cultivars. The method is sufficiently sensitive, reliable, and particularly suitable when estimations must be done in crude plant extract with low inhibitory activity.
- OSTI ID:
- 5745374
- Journal Information:
- Journal of Agricultural and Food Chemistry; (United States), Journal Name: Journal of Agricultural and Food Chemistry; (United States) Vol. 39:2; ISSN JAFCA; ISSN 0021-8561
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Tue Apr 09 00:00:00 EDT 1991
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·
OSTI ID:5509298
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OSTI ID:6290140
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BETA DECAY RADIOISOTOPES
CEREALS
CHEMICAL ANALYSIS
CHYMOTRYPSIN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
ENZYMES
GRAMINEAE
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEGUMINOSAE
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MAIZE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PLANTS
PROTEINS
RADIOISOTOPES
RELIABILITY
SEEDS
SENSITIVITY
SERINE PROTEINASES
TRACER TECHNIQUES
TRYPSIN
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BETA DECAY RADIOISOTOPES
CEREALS
CHEMICAL ANALYSIS
CHYMOTRYPSIN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
ENZYMES
GRAMINEAE
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEGUMINOSAE
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MAIZE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PLANTS
PROTEINS
RADIOISOTOPES
RELIABILITY
SEEDS
SENSITIVITY
SERINE PROTEINASES
TRACER TECHNIQUES
TRYPSIN