Early detection of degraded A14-/sup 125/I-insulin in human fibroblasts by the use of high performance liquid chromatography

Journal Article · Sun May 01 04:00:00 EDT 1983 · Diabetes; (United States)

OSTI ID:5745024

Stentz, F B; Harris, H L; Kitabchi, A E

We studied the metabolism of A14-125I-insulin in intact human fibroblasts using high performance liquid chromatography (HPLC) to detect and separate its early degradation products. The high resolving power of HPLC enabled us to separate what has been considered ''intact insulin'' by Sephadex G-50 chromatography or TCA precipitability into two additional peaks that had decreased biochemical properties with respect to immunoprecipitability and receptor binding but not decreased TCA precipitability. We conclude that human fibroblast is capable of metabolizing insulin within 2 min at 37 degrees C into intermediate molecules that can be detected by HPLC but not by TCA precipitability or molecular sieve chromatography.

• Research Organization: Department of Medicine, University of Tennessee Center for the Health Sciences, Memphis
• OSTI ID: 5745024
• Journal Information: Diabetes; (United States), Journal Name: Diabetes; (United States) Vol. 32:5; ISSN DIAEA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FIBROBLASTS
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
PATIENTS
PEPTIDE HORMONES
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES