Variation in hormone autonomy and regenerative potential of cells transformed by strain A66 of Agrobacterium tumefaciens

Journal Article · Tue Nov 30 23:00:00 EST 1982 · Cell; (United States)

DOI:https://doi.org/10.1016/0092-8674(82)90316-6· OSTI ID:5744801

Binns, A N ^[1]; Sciaky, D; Wood, H N

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1. Univ. of Pennsylvania, Philadelphia

Mutant Agrobacterium tumefaciens strain A66 is shown to differ from its wild-type progenitor (strain A6) by a spontaneous 2.7 kb DNA insert into the T-DNA region of its Ti plasmid. Tobacco stems transformed by A66 exhibit an attenuated response characterized by slow growth and shoot proliferation. Clonal analysis demonstrates that this response is due to an alteration in the growth and regenerative potential of transformed cells, rather than to variation in the frequency of fully autonomous cells within the primary tumor. Cloned A66 transformed tobacco cells exhibit an auxin requirement for growth that can be overcome by shoot proliferation. Other host species, however, may complement the A66 mutation yielding fully auxin-independent tumors when transformed by this bacterium.

• OSTI ID: 5744801
• Journal Information: Cell; (United States), Journal Name: Cell; (United States) Vol. 31; ISSN CELLB
• Country of Publication: United States
• Language: English

Related Subjects

553000* -- Agriculture & Food Technology
60 APPLIED LIFE SCIENCES
ANIMAL CELLS
AUXINS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
CELL CONSTITUENTS
CLONING
DNA
GROWTH
MICROORGANISMS
MUTATIONS
NICOTIANA
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PLANT CELLS
PLANT DISEASES
PLANT GROWTH
PLANT GROWTH REGULATORS
PLANT STEMS
PLANTS
PLASMIDS
RESPONSE MODIFYING FACTORS
TUMOR CELLS