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Kinetic characterization of /sup 13/NH/sub 3/ and (/sup 13/N)glutamine metabolism in rabbit heart

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5742168
; ; ; ; ; ; ;
The isolated arterially perfused rabbit interventricular septum was used to study the effects of methionine sulfoximine (MSO) on /sup 13/N-labeled ammonia (/sup 13/NH/sub 3/) and /sup 13/N-labeled glutamine ((/sup 13/N)) metabolism. Tissue time-activity curves were generated from bolus injections of /sup 13/NH/sub 3/ or (/sup 13/N)gln delivered interarterially during control conditions and in the presence of MSO. The fraction of /sup 13/N label retained in the tissue (EF(3)) was significantly less after a bolus of (/sup 13/N)gln than /sup 13/NH/sub 3/. MSO significantly decreased the EF(3) after a bolus of /sup 13/NH/sub 3/ or (/sup 13/N)gln. Chemical analyses using high-pressure liquid chromatography on tissue and effluent samples obtained after bolus injections of /sup 13/NH/sub 3/ and (/sup 13/N)gln with or without MSO present showed the following: 1) after a bolus of /sup 13/NH/sub 3/ or (/sup 13/N)gln the tissue /sup 13/N label is predominantly in (/sup 13/N)gln at 6 min after injection, and 2) MSO significantly decreases the synthesis of (/sup 13/N)gln after an /sup 13/NH/sub 3/ bolus. Thus it would appear that the glutamine synthetase reaction is primarily responsible for the retention of the /sup 13/N label from /sup 13/NH/sub 3/ as (/sup 13/N)gln in rabbit myocardium.
Research Organization:
Univ. of California, Los Angeles
DOE Contract Number:
AC03-76SF00012
OSTI ID:
5742168
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 246:1; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
AMINES
AMINO ACIDS
AMMONIA
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMISTRY
CHROMATOGRAPHY
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLUTAMINE
HEART
HYDRIDES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGASES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
METHIONINE
MINUTES LIVING RADIOISOTOPES
MUSCLES
MYOCARDIUM
NITROGEN
NITROGEN 13
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NITROGEN ISOTOPES
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PERFUSED ORGANS
RABBITS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES