Strain and sex differences in ozone toxicity in rats: Comparison with antioxidant concentrations

Conference · Sun Feb 25 23:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5740501

Slade, R; Crissman, K; Norwood, J; Hatch, G ^[1]

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1. Environmental Protection Agency, Research Triangle Park, NC (United States)

Sprague Dawley (SD) and Fischer 344(F) rats of both sexes were compared to determine if strain or sex differences in ozone (O{sub 3}) toxicity exist and whether these differences can be explained by basal antioxidant concentrations in bronchoalveolar lavage fluid (BALF) and BAL cells. Rats were exposed to 2.0 ppm O{sub 3} for 2 hours, and toxicity was measured as increased protein concentrations and % neutrophils in BALF. Following O{sub 3} exposure, the increase in neutrophils was 3.5 x greater, and the increase in BAL protein was 2.5 x greater in SD rats than in F rats. A positive correlation between BALF protein and neutrophils was noted for SD females, but not in other exposure groups. Differences were also found in BALF antioxidant concentrations. SD females has 2 to 4 x as much {alpha}-tocopherol (AT) in the BALF as the other animals. Males of both strains had about 50% more ascorbate (A) and urate (U) than females. In BAL cells, SD females had 3 x greater concentrations of AT, A and U than SD males. These results demonstrate that there are significant differences in the response of the two strains to O{sub 3} exposure. However, the large differences in ozone sensitivity do not appear to be explained by differences in BALF or BAL cell antioxidant concentrations.

• OSTI ID: 5740501
• Report Number(s): CONF-9004153--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 4:3; ISSN 0892-6638; ISSN FAJOE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIOXIDANTS
ASCORBIC ACID
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COMPARATIVE EVALUATIONS
CORRELATIONS
EVALUATION
LAVAGE
LEUKOCYTES
MAMMALS
MATERIALS
NEUTROPHILS
ORGANIC COMPOUNDS
OZONE
PROTEINS
RATS
RODENTS
SENSITIVITY
SEX DEPENDENCE
TOXICITY
VERTEBRATES
VITAMIN E
VITAMINS