Enhanced response to the induction of sister chromatid exchange by gamma radiation in neurofibromatosis
The study included 8 unrelated patients with neurofibromatosis, and 10 unrelated normal and healthy persons as controls. Whole blood samples were divided into plastic T flasks and exposed at room temperature to gamma rays. The radiation dose was 36 rad/minute, and the doses delivered were 0, 75, 150 and 300 rad. The lymphocytes were cultured in (RPMI) 1640 tissue culture medium and autologous serum (20%). Phytohemagglutinin and bromodeoxyuridine (Brdu) (10 microM) were added at initiation of culture and harvesting was done 64 to 68 hours after culture initiation. Slides were coded, differential staining was done, and sister chromatid exchanges (SCEs) and aberrations (gaps, breaks, dicentrics, fragments and minutes) were counted. In the controls no significant increase in frequency of SCE has been found (P greater than 0.5). In the patients, the frequencies significantly increased with the increase of dose of irradiation (P less than 0.001). Furthermore, after irradiation, the incidence of gaps, breaks, and dicentrics were significantly increased in patients compared with controls. Moreover, the incidence increased with the increase in the dose of radiation. The results are discussed with a conclusion that the results add to the indication of a genetic predisposition to develop cancer in neurofibromatosis patients.
- Research Organization:
- Mansoura Univ. Faculty of Medicine, Egypt
- OSTI ID:
- 5738888
- Journal Information:
- Cancer (Philadelphia); (United States), Vol. 10
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
RADIOSENSITIVITY
BLOOD
FIBROSIS
GAMMA RADIATION
NERVOUS SYSTEM DISEASES
PATIENTS
SISTER CHROMATID EXCHANGES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LEUKOCYTES
MATERIALS
MUTATIONS
PATHOLOGICAL CHANGES
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
560121* - Radiation Effects on Cells- External Source- (-1987)