The perfused isolated lung as a possible model for the study of lipid synthesis by type II cells in their natural environment

Journal Article · Sat Mar 01 04:00:00 EST 1980 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148009057513· OSTI ID:5736775

Batenburg, J J; Post, M; Oldenborg, V; Van Golde, L M

The incorporation of radioactively labeled palmitate and acetate into total and disaturated phosphatidylcholines was studied in the perfused whole lung, in surfactant secreted during perfusion, and in isolated alveolar type II cells. Exogenously added palmitate was found to be incorporated preferentially into the 2-position of total and disaturated phosphatidylcholines in all cases. Acetate, when supplied at a high concentration, was incorporated preferentially into the 2-position in all cases. However, acetate supplied at a low concentration was incorporated preferentially into the 2-position in type II cells and in surfactant, but preferentially into the 1-position in the whole lung. The dissimilarity in incorporation of acetate between isolated type II cells and perfused whole lung and the similarity in this respect between isolated type II cells and surfactant indicate that the perfused isolated lung may only be a good model for studying the synthesis of surfactant components by the type II cells in their natural environment if the products of processes in type II cells are separated from products of other cells after the perfusion. Both in surfactant and in lavaged lung tissue, labeled palmitate and acetate incorporated mainly into the 2-position of phosphatidylglycerol. This indicates that remodeling reactions are involved in the synthesis of dipalmitoylphosphatidylglycerol.

• OSTI ID: 5736775
• Journal Information: Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 1:1; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
BIOSYNTHESIS
BODY
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHOLINE
DRUGS
ESTERS
HEXADECANOIC ACID
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
LUNGS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
QUATERNARY COMPOUNDS
REACTION KINETICS
RESPIRATORY SYSTEM
RESPIRATORY TRACT CELLS
SYNTHESIS