Tolerance to cadmium and cadmium-binding ligands in Great Salt Lake brine shrimp (Artemia salina)

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Ecotoxicol. Environ. Saf.; (United States)

DOI:https://doi.org/10.1016/0147-6513(86)90026-6· OSTI ID:5736548

Jayasekara, S; Drown, D B; Sharma, R P

Information on the accumulation of cadmium in cytosolic proteins of Great Lake brine shrimp (Artemia salina) was obtained from animals collected directly from the lake and also from animal hatched and maintained in three sublethal concentrations of cadmium (0.5, 2.0, 5.0 ppm) in saltwater aquaria. Brine shrimp growth under these conditions was monitored by measuring body lengths during a 7-day exposure period. Heat-stable, cadmium-binding ligands were isolated and identified by Sephadex G-75 chromatography and atomic absorption spectrophotometry. Cadmium was found to be equally distributed between high and low molecular weight proteins in animals collected from the lake and the 0.5 ppm cadmium group. There was also a slight growth stimulation noted in the 0.5-pm group. Higher cadmium incorporation was noted in low molecular weight fractions with increasing cadmium concentration in the exposure media. Low molecular weight fractions were also found to have high uv absorption characteristics at 250 nm and low absorption at 280 nm. Molecular weight of the cadmium-binding ligands was found to be 11,000 as estimated by the gel filtration method. De novo synthesis of this protein was increased as a function of cadmium concentration in the media. However, slow accumulation of cadmium in other protein fractions was also noticed in higher cadmium exposure groups, suggesting the existence of possible tolerance mechanisms in brine shrimp exposed to suspected acute cadmium concentrations.

• Research Organization: Utah State Univ., Logan
• OSTI ID: 5736548
• Journal Information: Ecotoxicol. Environ. Saf.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ARTEMIA
CONTAMINATION
TOLERANCE
CADMIUM
BIOLOGICAL ACCUMULATION
ABSORPTION SPECTROSCOPY
CHEMICAL BONDS
METALLOTHIONEIN
MOLECULAR WEIGHT
PROTEINS
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
BRANCHIOPODS
CRUSTACEANS
ELEMENTS
INVERTEBRATES
METALLOPROTEINS
METALS
ORGANIC COMPOUNDS
SPECTROSCOPY
560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)