The effects of groundwater chemistry on co-metabolism of chlorinated solvents by methanotrophic bacteria

Conference · Mon Jan 01 04:00:00 EST 1990

OSTI ID:5735648

Palumbo, A V; Strandberg, G W ^[1]; Eng, W ^[2]

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1. Oak Ridge National Lab., TN (USA)
2. Tennessee Univ., Knoxville, TN (USA). Dept. of Microbiology

Degradation of chlorinated alkenes such as trichloroethylene (TCE) by methanotrophic bacteria is a promising technology for the remediation of contaminated groundwater. Ultimately, the success of this approach may be dependent on the influence of groundwater chemistry on degradation rates and extent. TCE can rapidly be reduced to low levels in laboratory cultures growing on defined media. However, if major changes in groundwater chemistry are necessary to achieve substantial TCE degradation, field application of processes (i.e., above ground and in situ treatment) may be limited by cost or logistic problems. For example, the presence of competitive inhibitors may limit the extent of TCE degradation. The goal of our research is to quantify the potential effects of groundwater chemistry on the biodegradation of TCE by methanotrophs and to define concentrations of methane that need to be added to the system to produce maximum rates of TCE degradation. This includes evaluation of major nutritional requirements (e.g., PO{sub 4}) in addition to the focus on competitive inhibition. 20 refs., 6 figs.

• Research Organization: Oak Ridge National Lab., TN (USA)
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5735648
• Report Number(s): CONF-900402-15; ON: DE91014223
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540350 -- Environment
Aquatic-- Site Resource & Use Studies-- (1990-)
550500* -- Metabolism
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKANES
AMMONIA
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHEMISTRY
CHLORINE COMPOUNDS
DECOMPOSITION
GEOCHEMISTRY
GROUND WATER
HALOGEN COMPOUNDS
HYDRIDES
HYDROCARBONS
HYDROGEN COMPOUNDS
METABOLISM
METHANE
METHANOTROPHIC BACTERIA
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NUTRIENTS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLLUTANTS
SOLVENTS
WATER