Determination of morphological parameters of biological cells by analysis of scattered-light distributions
The extraction of morphological parameters from biological cells by analysis of light-scatter patterns is described. A light-scattering measurement system has been designed and constructed that allows one to visually examine and photographically record biological cells or cell models and measure the light-scatter pattern of an individual cell or cell model. Using a laser or conventional illumination, the imaging system consists of a modified microscope with a 35 mm camera attached to record the cell image or light-scatter pattern. Models of biological cells were fabricated. The dynamic range and angular distributions of light scattered from these models was compared to calculated distributions. Spectrum analysis techniques applied on the light-scatter data give the sought after morphological cell parameters. These results compared favorably to shape parameters of the fabricated cell models confirming the mathematical model procedure. For nucleated biological material, correct nuclear and cell eccentricity as well as the nuclear and cytoplasmic diameters were determined. A method for comparing the flow equivalent of nuclear and cytoplasmic size to the actual dimensions is shown. This light-scattering experiment provides baseline information for automated cytology. In its present application, it involves correlating average size as measured in flow cytology to the actual dimensions determined from this technique. (ERB)
- Research Organization:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5734330
- Report Number(s):
- LA-8143-T
- Resource Relation:
- Other Information: Thesis
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CYTOLOGICAL TECHNIQUES
MEASURING INSTRUMENTS
DENSITOMETERS
PERFORMANCE TESTING
ERYTHROCYTES
MORPHOLOGY
MEASURING METHODS
CELL FLOW SYSTEMS
COMPARATIVE EVALUATIONS
CORRELATIONS
EXPERIMENTAL DATA
ISOLATED VALUES
LIGHT SCATTERING
PHOTOGRAPHIC FILMS
SIZE
STRUCTURAL MODELS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DATA
DATA FORMS
INFORMATION
MATERIALS
NUMERICAL DATA
PHOTOMETERS
TESTING
550300* - Cytology