Comparison of protein patterns of xrs-5, a radiosensitive Chinese hamster ovary cell line, and CHO-K1, its radioresistant parent, using two-dimensional gel-electrophoresis

Conference · Tue Jan 01 04:00:00 EST 1991

OSTI ID:5734047

Kramer, J M ^[1]

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1. Miami Univ., Oxford, OH (USA). Dept. of Zoology

X-ray sensitive strains of Chinese hamster ovary cell lines have been used to analyze radiation repair mechanisms. One cell line, xrs-5, has been shown to be very sensitive to ionizing radiation and radical forming chemical mutagens. This sensitivity is thought to be a result a mutation in the DNA double strand break (DSB) repair mechanism, and its characterization has been a goal of several repair mechanism studies. Using two-dimensional gel electrophoresis, we have detected a protein (MW approximately 55KD) in the DNA/Nuclear Matrix (nucleoid) cell fraction of CHO-Kl cells that is absent in the nucleoid fraction of xrs-5. This protein is present, however, in both CHO-Kl and xrs-5 whole cell protein maps. To determine whether the 55KD protein is responsible for the radiosensitive and defective DSB repair phenotype of xrs-5 cells, studies are now underway to analyze revertants of xrs-5 that are proficient in DSB repair. Furthermore, an effort to sequence the protein in question is planned. 23 refs., 2 figs.

• Research Organization: Argonne National Lab., IL (USA)
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5734047
• Report Number(s): ANL/CP-73075; CONF-9103136--7; ON: DE91013771
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
DISTRIBUTION
DNA REPAIR
ELECTROPHORESIS
MOLECULAR STRUCTURE
MUTAGENESIS
MUTANTS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
REVERTANTS
STRAND BREAKS
SUBCELLULAR DISTRIBUTION
SURVIVAL CURVES
TWO-DIMENSIONAL ELECTROPHORESIS