Nature of the oxidation-reduction properties of nitrite reductase from Desulfovibrio desulfuricans

Journal Article · Tue Sep 16 04:00:00 EDT 1980 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(80)91211-5· OSTI ID:5729634

Liu, M C; DerVartanian, D V; Peck, Jr, H D

Nitrite reductase as isolated from Desulfovibrio desulfuricans shows a complex set of rhombically distorted high-spin ferric heme and low-spin ferric heme resonances at 11/sup 0/K. These resonances disappear on enzymatic reduction with hydrogen, hydrogenase from D. vulgaris and FAD as redox mediator. The addition of nitrite to the reduced enzyme results in reoxidation of nitrite reductase as evidenced by the reappearance of most of the initial signal intensities of high-spin and low-spin ferric heme resonances. Simultaneously an intense and unusual broadened signal appears in the g = 2 region, suggesting the formation of a novel heme-nitric oxide signal with the main g-value at 2.08. Confirmation of this heme-nitric oxide complex was demonstrated by reoxidation of reduced enzyme with /sup 15/NO/sub 2//sup -1/ instead of /sup 14/NO/sub 2//sup -1/ resulting in a decrease from three to two of the hyperfine interaction pattern of nitrogen. Thus nitrite reduction to ammonia by nitrite reductase occurs via a heme-nitric oxide intermediate as reported with spinach nitrite reductase.

• Research Organization: Univ. of Georgia, Athens
• DOE Contract Number: AS09-79ER10499
• OSTI ID: 5729634
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Journal Issue: 1 Vol. 96:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMMONIA
BACTERIA
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMICAL PROPERTIES
CHEMICAL REACTIONS
COMPLEXES
DESULFOVIBRIO
ELECTRON SPIN RESONANCE
ENZYMES
HEME
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDRIDES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NITRIC OXIDE
NITRITES
NITROGEN 14
NITROGEN 15
NITROGEN COMPLEXES
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NITROGEN ISOTOPES
NITROGEN OXIDES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDATION
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
REDUCTION
RESONANCE
SODIUM COMPOUNDS
STABLE ISOTOPES
TRACER TECHNIQUES