Proteins of the rat prostate. II. Synthesis of new proteins in the ventral lobe during castration-induced regression
Ventral prostates from adult Sprague-Dawley rats at different days postcastration were cut into one to two mm.3 pieces and incubated in medium containing S-35-methionine (100 uCi/ml.) at 37 C under 95% oxygen and 5% carbon dioxide for four hours. The incubated tissues were subjected to two-dimensional electrophoresis and radiofluorography. Over 100 spots were developed in the fluorograms. Three groups of spots, representing cytoskeletal proteins, androgen-dependent proteins and castration-induced proteins, were further evaluated by a computer-based densitometer. The level of densitometry absorption is proportional to the amount of radioactivity in each spot. The synthesis of cytoskeletal proteins, such as actin and tropomyosin, were relatively constant throughout the course of prostatic regression. The rate of synthesis of androgen-dependent proteins declined rapidly from a high level of synthesis before castration to a non-detectable level by Day 3 postcastration. However, three proteins, which were either not synthesized (spot G and spot H) or synthesized at a very low level (spot I) before castration, were the major proteins synthesized by the prostate during early stages of its regression. The rate of synthesis of these proteins reached a peak by Day 4 postcastration, declined rapidly and remained at a low level thereafter. The respective molecular weights and isoelectric points for these three proteins were 33 Kd and 7.2 for spot G, 38 Kd and 5.3 for spot H and 64 Kd and 6.0 for spot I. Previous findings showed that prostatic regression in rats was associated with a surge of activities in proteolytic enzymes which peaked five to six days postcastration.
- Research Organization:
- Northwestern Univ. Medical School, Chicago IL
- OSTI ID:
- 5727773
- Journal Information:
- J. Urol.; (United States), Journal Name: J. Urol.; (United States) Vol. 138:4; ISSN JOURA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANDROGENS
ANDROSTANES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOMEDICAL RADIOGRAPHY
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FLUOROSCOPY
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MALE GENITALS
MAMMALS
MEDICINE
METHIONINE
NUCLEAR MEDICINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROSTATE
PROTEINS
RADIOISOTOPES
RADIOLOGY
RATS
RODENTS
STEROID HORMONES
STEROIDS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TESTOSTERONE
TISSUE CULTURES
TRACER TECHNIQUES
VERTEBRATES