Histochemical study of brown-fat cells in the golden hamster (Mesocricetus auratus) in cultures
The authors undertake the task of studying the synthesis of certain hormones by brown-fat cells. The authors used brown-fat cells from the golden hamster. The metabolism of brown-fat cells was studied on precultured cells, which made it possible to detect the synthesis of the studied substances rather than their accumulation in the organ. The authors conducted three experiments. First, fragments of brown fat were cultivated in diffusion chambers in vivo. Pieces of brown fat were cultivated in parallel in vitro on agar (organotypic cultures) and on plasma (histotypic cultures). During cultivation in diffusion chambers, the chambers were implanted in the abdominal cavity of young white rats. For in vitro cultivation, TCM 199 plus 15-20% calf serum was used. A total of 36 cultures with 12 cultures in each series of experiments were performed. The auto-radiographic studies of brown-fat cells were conducted on 24-hour cultures and on brown-fat fragments taken from the intact animal. The cultures were incubated with isotopes for 1 h. Either (/sup 3/H)lysine (87.3 Ci/mM specific activity), (/sup 3/H)arginine (16.7 Ci/mM), (/sup 3/H)glycerol (43 Ci/mM), or (/sup 3/H)cholesterol (43 Ci/mM) were added to the medium. After incubation, the cultures were washed three times in pure medium, fixed in Sierra fluid, and embedded in paraffin. The paraffin sections were covered with Ilford K/sub 2/ emulsion, and the preparations were exposed for 20 days at 4/sup 0/C temperature. Radio-immunological methods were used to study the accumulation of estradiol-17-beta in the culture medium by the Dobson method and that of testerone. The culture medium was taken on cultivation days 2,4,6,8, and 10. The medium was changed during cultivation every third day, which made it possible to judge the rates of accumulation of material with increase in the cultivation times.
- Research Organization:
- A. N. Severtsov Institute of Evolutionary Morphology and Animal Ecology, Moscow, USSR
- OSTI ID:
- 5726713
- Journal Information:
- Dokl. Biol. Sci. (Engl. Transl.); (United States), Vol. 282:1-6; Other Information: Translated from Dokl. Akad. Nauk SSSR; 282: No. 1, 168-170(May 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ESTRADIOL
BIOSYNTHESIS
RADIOIMMUNOASSAY
FAT CELLS
METABOLISM
TESTOSTERONE
ADIPOSE TISSUE
ARGININE
AUTORADIOGRAPHY
CELL CULTURES
CHOLESTEROL
EOSIN
GLYCEROL
HAMSTERS
INCUBATION
LABELLING
LYSINE
RADIOACTIVITY
TRITIUM COMPOUNDS
ALCOHOLS
AMINO ACIDS
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DYES
ESTRANES
ESTROGENS
HORMONES
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INDICATORS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
STEROLS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550301* - Cytology- Tracer Techniques
550501 - Metabolism- Tracer Techniques