Oxalic acid biosynthesis and oxalacetate acetylhydrolase activity in Streptomyces cattleya

Journal Article · Sun Nov 15 04:00:00 EST 1987 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(87)90470-X· OSTI ID:5726455

Houck, D R; Inamine, E

In addition to producing the antibiotic thienamycin, Streptomyces cattleya accumulates large amounts of oxalic acid during the course of a fermentation. Washed cell suspensions were utilized to determine the specific incorporation of carbon-14 into oxalate from a number of labeled organic and amino acids. L-(U-/sup 14/C)aspartate proved to be the best precursor, whereas only a small percentage of label from (1,5-/sup 14/C)citrate was found in oxalate. Cell-free extracts catalyzed the formation of (/sup 14/C)oxalate and (/sup 14/C)acetate from L-(U-/sup 14/C)aspartate. When L-(4-/sup 14/C)aspartate was the substrate only (/sup 14/C)acetate was formed. The cell-free extracts were found to contain oxalacetate acetylhydrolase, the enzyme that catalyzes the hydrolysis of oxalacetate to oxalate and acetate. The enzyme is constitutive and is analogous to enzymes in fungi that produce oxalate from oxalacetate. Properties of the crude enzyme were examined.

• Research Organization: Merck, Sharp and Dohme Research Laboratories, Rahway, NJ
• OSTI ID: 5726455
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 259:1; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ASPARTIC ACID
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHEMICAL PROPERTIES
CITRATES
DICARBOXYLIC ACIDS
ENZYME ACTIVITY
ENZYMES
FERMENTATION
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXALATES
OXALIC ACID
STREPTOMYCES
SYNTHESIS
TRACER TECHNIQUES