Quantitative surface studies of protein adsorption by infrared spectroscopy. II. Quantification of adsorbed and bulk proteins
Journal Article
·
· Anal. Biochem.; (United States)
Attenuated total reflectance Fourier transform infrared spectra of surface-adsorbed proteins are correlated with concentration measurements determined by /sup 125/I-labeled proteins. This paper demonstrates that linear correlations between the intensity of the major bands of proteins and the quantity of proteins can be obtained for human albumin and immunoglobulin G up to surface concentrations of approximately 0.25 microgram/cm2. A poorer correlation was observed for human fibrinogen. A linear correlation was also observed between the concentration in the bulk solution and the major bands of albumin up to a concentration of 60 mg/ml.
- Research Organization:
- Bio-Integration, Inc., Columbus, OH
- OSTI ID:
- 5726064
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 165:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADSORPTION
ALBUMINS
BETA DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FIBRINOGEN
FOURIER ANALYSIS
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNOGLOBULINS
INFRARED SPECTRA
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SORPTION
SPECTRA
SURFACE PROPERTIES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ADSORPTION
ALBUMINS
BETA DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FIBRINOGEN
FOURIER ANALYSIS
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNOGLOBULINS
INFRARED SPECTRA
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SORPTION
SPECTRA
SURFACE PROPERTIES
TRACER TECHNIQUES