GABA/sub A/ receptor-controlled /sup 36/Cl/sup -/ influx in cultured rat cerebellar granule cells
..gamma..-Aminobutyric acid (GABA)-mediated and bicuculline-sensitive /sup 36/Cl/sup -/ influx and bicuculline-sensitive (/sup 3/H) GABA binding were demonstrated in cultures of rat cerebellar granule cells. The addition of 10/sup -5/ M GABA produced a two-fold increase in /sup 36/Cl/sup -/ influx over the basal level and the maximal increase was observed after approximately 20 sec. Progressive occupation of GABA/sub A/ receptor by (/sup 3/H)-(1S-9R)-bicuculline methiodide decreased /sup 36/Cl/sup -/ influx activated by 10 ..mu..M GABA. The above results suggest that primary cultures of rat cerebellar granule cells provide a new and reliable model for studying the GABA activated chloride fluxes. 41 references, 4 figures.
- Research Organization:
- Central Research Institute for Chemistry, Budapest, Hungary
- OSTI ID:
- 5726032
- Journal Information:
- Life Sci.; (United States), Vol. 41:3
- Country of Publication:
- United States
- Language:
- English
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AMINOBUTYRIC ACID
BIOCHEMICAL REACTION KINETICS
RECEPTORS
CHLORIDES
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ANIMAL CELLS
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BIOLOGICAL MODELS
BRAIN
CELL CULTURES
CHLORINE 36
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
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CHLORINE COMPOUNDS
CHLORINE ISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALIDES
HALOGEN COMPOUNDS
IONS
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ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
ODD-ODD NUCLEI
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ORGANIC COMPOUNDS
ORGANS
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550201* - Biochemistry- Tracer Techniques