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Study of ketone body kinetics in children by a combined perfusion of /sup 13/C and /sup 2/H3 tracers

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5724749
;

Ketone body kinetics were quantified in six children (3-5 yr old), who were fasted for 13-22 h, by a combined perfusion of (3-/sup 13/C)acetoacetate ((/sup 13/C)AcAc) and D-(-)-beta-(4,4,4-/sup 2/H3)hydroxybutyrate (beta-(/sup 2/H3)OHB) and gas chromatography-mass spectrometry analysis. Results were analyzed according to the single-pool (combined enrichments) or the two-accessible pools models. After 20-22 h of fasting, ketone body turnover rate was 30-50 mumol.kg-1.min-1, a rate achieved after several days of fasting in adults. At low ketosis, acetoacetate was the ketone body preferentially synthesized de novo and utilized irreversibly. When ketosis increased, acetoacetate irreversible disposal was not enhanced, since it was largely converted into beta-OHB, whereas beta-OHB irreversible disposal was very much increased. The single-pool and two-pool models gave similar ketone body turnover rates when (/sup 13/C)AcAc was the tracer, whereas the use of beta-(/sup 2/H3)OHB gave some more divergent results, especially at low ketosis. These studies demonstrate that ketogenesis is very active in short-term fasted children and that the use of a combined infusion of (/sup 13/C)AcAc and beta-(/sup 2/H3)OHB is a convenient way to give insight into individual ketone body kinetics.

Research Organization:
Institut National de la Sante et de la Recherche Medicale U 188, Paris, France
OSTI ID:
5724749
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETOACETATES
AGE GROUPS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACID SALTS
CHILDREN
CHROMATOGRAPHY
DEUTERIUM
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
FASTING
GAS CHROMATOGRAPHY
GLANDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LIGHT NUCLEI
LIVER
MASS SPECTROSCOPY
METABOLISM
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES