Distribution of physostigmine and metabolites in brain subcellular fractions of the rat
The distribution of /sup 3/H-physostigmine (Phy) has been studied in the rat brain subcellular fractions at various time intervals following i.v. injection. /sup 3/H-Phy or its metabolites rapidly accumulate into the cytoplasm of cells and penetrates the intracellular compartments. Kinetic studies of the subcellular distribution of radioactivity (RA) per gm of rat brain following i.v. injection of /sup 3/H-Phy show peak concentrations at 30 min in all subcellular fractions with the exception of mitochondria. In the mitochondrial fraction the RA levels continue to rise from 4682 +/- 875 DPM/gm at 5 min to 27,474 +/- 2825 DPM/gm at 60 min (P < .05). The cytosol contains the highest RA: 223,341 +/- 21,044 DPM/gm at 30 min which declined to 53,475 +/- 3756 DPM/gm at 60 min. RA in synaptosome, microsomes and myelin increases from 5 to 30 min, and declines at 60 min. In vitro studies did not show a greater uptake of RA by the mitochondrial or synaptosomal fractions. The finding of relatively high concentrations of RA in the mitochondrial fraction at 60 min increases the likelihood that Phy or its metabolites could interfere with the physiological function of the organelle. 21 references, 1 figure, 2 tables.
- Research Organization:
- Southern Illinois Univ. School of Medicine, Springfield
- OSTI ID:
- 5724664
- Report Number(s):
- CONF-870369-; TRN: 88-005706
- Journal Information:
- Life Sci.; (United States), Vol. 4:17; Conference: Federation of American Societies for Experimental Biology conference, Washington, DC, USA, 29 Mar 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PARASYMPATHOMIMETICS
SUBCELLULAR DISTRIBUTION
BRAIN
CYTOPLASM
EXPERIMENTAL DATA
INTRAVENOUS INJECTION
METABOLITES
MICROSOMES
MITOCHONDRIA
MYELIN
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
DATA
DISTRIBUTION
DRUGS
INFORMATION
INJECTION
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
NERVOUS SYSTEM
NUMERICAL DATA
ORGANOIDS
ORGANS
RODENTS
VERTEBRATES
550501* - Metabolism- Tracer Techniques