Binding of decomposition products of UDP-galactose to the microsomes and polyribosomes isolated from rat liver

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Biochem. Med. Metab. Biol.; (United States)

DOI:https://doi.org/10.1016/0885-4505(87)90074-0· OSTI ID:5724619

Kopacz-Jodczyk, T; Galasinski, W

UDP-D-(U-/sup 14/C)galactose is decomposed to (U-/sup 14/C)galactose-1-phosphate and (U-/sup 14/C)galactose by rat liver microsomal and crude polyribosomal fractions, under conditions commonly used to assay of glycosyltransferase activities. UDP-D-(U-/sup 14/C)galactose, at neutral pH, is also chemically degraded to the (U-/sup 14/C)galactose-1,2-cyclic phosphate. The 1,2-cyclic phosphate derivative of galactose also exists in the commercial UDP-D-(U-/sup 14/C)galactose. It is a very important finding that products of the UDP-D-(U-/sup 14/C)galactose decomposition are tightly, although nonenzymatically, bound to tested subcellular fractions and may create a false impression of protein glycosylation. The application of controls containing all radioactive substances present in suitable samples is recommended in order to avoid incorrect interpretations of the results.

• Research Organization: Institute of Chemistry, Bialystok, Poland
• OSTI ID: 5724619
• Journal Information: Biochem. Med. Metab. Biol.; (United States), Vol. 38:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GALACTOSE
BIOCHEMICAL REACTION KINETICS
METABOLISM
CARBON 14 COMPOUNDS
IN VITRO
LIVER
MICROSOMES
PHOSPHORIC ACID ESTERS
RATS
RIBOSOMES
TRACER TECHNIQUES
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ESTERS
GLANDS
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
REACTION KINETICS
RODENTS
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques