Binding of decomposition products of UDP-galactose to the microsomes and polyribosomes isolated from rat liver
UDP-D-(U-/sup 14/C)galactose is decomposed to (U-/sup 14/C)galactose-1-phosphate and (U-/sup 14/C)galactose by rat liver microsomal and crude polyribosomal fractions, under conditions commonly used to assay of glycosyltransferase activities. UDP-D-(U-/sup 14/C)galactose, at neutral pH, is also chemically degraded to the (U-/sup 14/C)galactose-1,2-cyclic phosphate. The 1,2-cyclic phosphate derivative of galactose also exists in the commercial UDP-D-(U-/sup 14/C)galactose. It is a very important finding that products of the UDP-D-(U-/sup 14/C)galactose decomposition are tightly, although nonenzymatically, bound to tested subcellular fractions and may create a false impression of protein glycosylation. The application of controls containing all radioactive substances present in suitable samples is recommended in order to avoid incorrect interpretations of the results.
- Research Organization:
- Institute of Chemistry, Bialystok, Poland
- OSTI ID:
- 5724619
- Journal Information:
- Biochem. Med. Metab. Biol.; (United States), Journal Name: Biochem. Med. Metab. Biol.; (United States) Vol. 38:2; ISSN BMMBE
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ESTERS
GALACTOSE
GLANDS
HEXOSES
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
MICROSOMES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHORIC ACID ESTERS
RATS
REACTION KINETICS
RIBOSOMES
RODENTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES