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Glutaraldehyde pretreatment blocks temperature-induced high-affinity (/sup 3/H) tryptamine binding

Journal Article · · Life Sci.; (United States)
;
The effect of glutaraldehyde (and Azure A) on temperature-sensitive high-affinity (/sup 3/H) tryptamine binding was investigated in rat brain synaptic plasma membranes. In the 0.01-0.1 % concentration range, the glutaraldehyde pretreatment preferentially inhibited only the above-mentioned portion of the binding, whereas the posttreatment of this reagent had no effect. On the other hand, in cases of pretreatment or posttreatment, a concentration of glutaraldehyde as high as 0.1 % was inactive on the basal (/sup 3/H) ligand binding capacity of the membranes. Furthermore, it was revealed that the Scatchard plot of (/sup 3/H) tryptamine binding in membranes pretreated with glutaraldehyde conformed to a straight line, as did a similar plot of temperature-independent binding. And, it was interesting to find that the binding parameters (K/sub D/ and B/sub max/ values) of both samples corresponded closely to each other. On the contrary, in all concentrations, Azure A affected nonspecifically both the temperature-dependent and the independent (/sup 3/H) tryptamine binding to the same degree, regardless of whether or not there was pretreatment or posttreatment. 17 references, 2 figures, 1 table.
Research Organization:
Josai Univ., Saitama, Japan
OSTI ID:
5723223
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:2; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALDEHYDES
AMINES
ANIMALS
AROMATICS
AZAARENES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
BRAIN
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
HETEROCYCLIC COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
RATS
REACTION KINETICS
REAGENTS
RECEPTORS
RODENTS
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPTAMINES
VERTEBRATES