Activity of cAMP-dependent protein kinases and cAMP-binding proteins of rat kidney cytosol during dehydration

Journal Article · Fri Sep 20 04:00:00 EDT 1985 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5722400

Zelenina, M N; Solenov, E I; Ivanova, L N

The activity of cAMP-dependent protein kinases, the binding of cAMP, and the spectrum of cAMP-binding proteins in the cytosol of the renal papilla was studied in intact rats and in rats after 24 h on a water-deprived diet. It was found that the activation of protein kinases by 10/sup -6/ M cAMP is significantly higher in the experimental animals than in the intact animals. In chromatography on DEAE-cellulose, the positions of the peaks of specific reception of cAMP corresponded to the peaks of the regulatory subunits of cAMP-dependent protein kinases of types I and II. In this case, in intact animals more than 80% of the binding activity was detected in peaks II, whereas in rats subjected to water deprivation, more than 60% of the binding was observed in peak I. The general regulatory activity of the cytosol was unchanged in the experimental animals in comparison with intact animals. It is suggested that during dehydration there is an induction of the synthesis of the regulatory subunit of type I cAMP-dependent protein kinase in the renal papilla.

• Research Organization: Institute of Cytology and Genetics, Novosibirsk, USSR
• OSTI ID: 5722400
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:3; ISSN BIORA
• Country of Publication: United States
• Language: English

Related Subjects

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550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CELL CONSTITUENTS
CHROMATOGRAPHY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DEHYDRATION
ENZYME ACTIVITY
ENZYMES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIQUID SCINTILLATORS
MAMMALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RADIORECEPTOR ASSAY
RATS
RODENTS
SCINTILLATION COUNTING
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES