Kinetic study and mathematical modeling of methanogenesis of acetate using pure cultures of methanogens

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260300510· OSTI ID:5722220

Yang, S T; Okos, M R

Kinetics of methanogenesis from acetate was studied using pure cultures of Methanosarcina barkeri and Methanosarcina mazei. Methane formation was found to be associated with cell growth. Nearly equimolar methane was produced from acetate during the methanogenic growth, and about 1.94 g of cells were formed from each mole of acetate consumed. Cell growth can be estimated from methane production. Significant substrate inhibition was found when acetate concentration was higher than 0.12 M. Among the three methanogenic strains studied, M. mazei strain 56 had the highest specific growth rate at all acetate concentrations studied and was least sensitive to environmental factors investigated (e.g., acetate concentration). The maximum specific growth rate found for strain S6 was 0.022/hour at acetic acid concentration around 7 g/L. The other two strains studied were M. barkeri strain 227 and strain MS. Growth of M. barkeri was completely inhibited at sodium acetate concentrations higher than 0.24 M. The maximum specific growth rate found for strains 227 and MS was 0.019 and 0.021/hour at acetic acid concentrations of 3.6 and 6.8 g/L, respectively. A kinetic model with substrate inhibition was developed and can be used to simulate the methane formation from M. mazei strain S6 grown on acetate at 35 degrees C, pH 7. (Refs. 29).

• Research Organization: Purdue Univ., West Lafayett, IN 47907. Dept. Agricultural Engineering
• OSTI ID: 5722220
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 30:5; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
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ACETATES
ALKANES
ANAEROBIC DIGESTION
BACTERIA
BIOCONVERSION
CARBOXYLIC ACID SALTS
CHEMICAL REACTION KINETICS
DIGESTION
GAS YIELDS
GROWTH
HYDROCARBONS
INHIBITION
KINETICS
MANAGEMENT
MESOPHILIC CONDITIONS
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
PH VALUE
PROCESSING
REACTION KINETICS
SUBSTRATES
WASTE MANAGEMENT
WASTE PROCESSING
YIELDS