Bacterial chemotactic oligopeptides and the intestinal mucosal barrier

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Gastroenterology; (United States)

OSTI ID:5722129

Ferry, D M; Butt, T J; Broom, M F; Hunter, J; Chadwick, V S

Intestinal absorption and enterohepatic circulation of N-formyl-methionyl-leucyl-/sup 125/I-tyrosine, a bioactive synthetic analog of the bacterial chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine has been investigated in the rat. In ileum and proximal and distal colon, dithiothreitol, which increases mucosal permeability, increased peptide absorption and biliary recovery fourfold, 70-fold, and 20-fold over control values, respectively. When dithiothreitol was combined with d-l-benzyl succinate, a potent inhibitor of intestinal carboxypeptidase, absorption and biliary recovery from ileal loops increased markedly to 40-fold over control, whereas there was no further increase in absorption from colon loops. There was a strong correlation between biliary N-formyl-methionyl-leucyl-/sup 125/I-tyrosine recovery and intestinal absorption of /sup 51/Cr-ethylenediaminetetraacetate, a marker of passive mucosal permeability (r = 0.97). We conclude that in the ileum both enzymic degradation and restricted mucosal permeability contribute to the intestinal barrier to luminal bacterial formyl oligopeptides. In the colon, however, enzymic mechanisms are less active and restricted mucosal permeability is the major factor. Abnormalities of the intestinal mucosal barrier to proinflammatory bacterial peptides could play a role in inflammatory disorders of the gut.

• Research Organization: Univ. of Otago Medical School, Dunedin (New Zealand)
• OSTI ID: 5722129
• Journal Information: Gastroenterology; (United States), Journal Name: Gastroenterology; (United States) Vol. 97:1; ISSN GASTA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BILE
BIOLOGICAL MARKERS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMIUM 51
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DIGESTIVE SYSTEM DISEASES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
HYDROXY ACIDS
INFLAMMATION
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEMBRANES
MUCOUS MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PEPTIDES
PERMEABILITY
PHENYLALANINE
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
TYROSINE
UPTAKE
VERTEBRATES