Sucrose synthesis in gamma irradiated sweet potato
Effect of ..cap alpha..-irradiation carbohydrate metabolism was examined to elucidate mechanism of sucrose accumulation in sweet potato (SP). Enzymes examined were: ..beta..-amylase, phosphorylase, phosphoglucomutase, phosphoglucose isomerase, sucrose phosphate synthetase and sucrose synthetase. Irradiated SP (Red Jewell) sucrose was synthesized to yield 10.7% after 4 d PI. Activities of sugar synthesizing enzymes in irradiated SP were enhanced to different degrees using 100-200 Krad and 3 d PI at 24/sup 0/C. Phosphorylase and phosphoglucomutases specific activities reached 2.4 and 1.8 folds, respectively compared to control SP. ..beta..-amylase, phosphoglucose isomerase, sucrose synthetase and sucrose phosphate synthetase were also affected to yield 1.2, 1.3, 1.3 and 1.2 folds, respectively compared to controls. It is believed that amylase hydrolyzed starch to glucose which is converted to fructose by phosphoglucose isomerase. Sucrose is then formed by sucrose phosphate synthetase and/or sucrose synthetase leading to its accumulation. The irradiated SP was used for alcohol fermentation leading to 500 gal. of 200 proof ethanol/acre (from 500-600 bushels tuber/acre).
- Research Organization:
- Univ. of Georgia, Athens
- OSTI ID:
- 5722011
- Report Number(s):
- CONF-8611204-
- Journal Information:
- Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 184:4; ISSN PSEBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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560140 -- Radiation Effects on Plants
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AMYLASE
BIOCONVERSION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
DISACCHARIDES
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ETHANOL
FERMENTATION
FOOD
GAMMA RADIATION
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
IONIZING RADIATIONS
ISOMERASES
LIGASES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLANTS
POTATOES
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
SACCHAROSE
SYNTHESIS
TRANSFERASES
TUBERS
VEGETABLES
YIELDS