Characteristics of poly(A)-containing nuclear informosomes from wheat germ and the proteins contained in them
Purified preparations of poly(A)-containing nuclear informosomes from wheat germ and their poly(A)-protein fragments were isolated by chromatography on oligo (dT)-cellulose. Poly(A)-containing informosomes account for approximately 15% of the radioactivity of the rapidly labelled RNA of the nuclear extract; they have sedimentation coefficients from 10 to 80S and a buoyant density in a CsCl gradient of 1.40 g/cm/sup 3/. Electrophoresis of the proteins of poly(A)-containing nuclear informosomes in polyacrylamide gel with SDS revealed two major polypeptides with M/sub r/ 34,000 and 52,000 and minor polypeptides with M/sub r/ 16,000, 23,000, 64,000 and 68,000. Nuclear informosomes contain protein-bound poly(A)-sequences (50-100 nucleotides long) resistant to pancreatic and Tl RNases. Poly(A)-protein fragments have a buoyant density in CsCl of 1.38 g/cm/sup 3/, sedimentation coefficients of 4-12S in ribonucleoprotein form, and of 3-4S after deproteination. Poly(A)-protein complexes contain polypeptides with M/sub r/ 52,000, 64,000 and 68,000, the two with M/sub r/ 52,000 and 64,000 being most tightly bound to poly(A).
- Research Organization:
- Institute of Molecular Biology and Biochemistry, Alma-Ata, USSR
- OSTI ID:
- 5721841
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 50:8; Other Information: Translated from Biokhimiya; 50: No. 8, 1323-1329(Aug 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
BIOCHEMISTRY
PRECURSOR
TRACER TECHNIQUES
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ADENOSINE
CELL NUCLEI
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ELECTROPHORESIS
ENZYME ACTIVITY
LABELLING
PLANT CELLS
POLYPEPTIDES
RNA-ASE
SEDIMENTATION
TRITIUM COMPOUNDS
URIDINE
WHEAT
ALKALI METAL COMPOUNDS
AZINES
CELL CONSTITUENTS
CEREALS
CESIUM COMPOUNDS
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
ENZYMES
ESTERASES
GRASS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PHOSPHODIESTERASES
PLANTS
PYRIMIDINES
RIBOSIDES
URACILS
550401* - Genetics- Tracer Techniques
550301 - Cytology- Tracer Techniques