(The genetic and molecular biology of hydrogen metabolism in sulfate reducing bacteria): Progress report

Technical Report · Tue Dec 01 04:00:00 EST 1987

OSTI ID:5720780

Wall, J D

Studies aimed at developing methods to exploit the genetics and molecular biology of the sulfate-reducing bacteria are described. The long-term goal of these studies is to examine the H/sub 2/ metabolism of Desulfovibrio desulfuricans. We recently observed transduction mediated by an apparently defective bacteriophage. The requirements for transduction, the efficiency of cultures as donors or recipients and the kinetics of production of the phage have been examined. Since it is sometimes necessary to manipulate the bacterial cultures outside the anaerobic chamber, it seemed pertinent to establish the oxygen tolerance of the D. desulfuricans cultures. We observed dramatic morphological responses of colonies upon exposure to air. The development of a genetic exchange process was predicated on the facility of handling these bacteria during standard microbiological procedures. Experience with these bacteria has revealed that for D. desulfuricans ATCC 27774, the doubling time in the standard lactate/sulfate based medium at 31 C is 6 to 9 hours and that 7 to 9 days is required to observe good colony formation.

• Research Organization: Missouri Univ., Columbia (USA)
• DOE Contract Number: FG02-87ER13713
• OSTI ID: 5720780
• Report Number(s): DOE/ER/13713-1; ON: DE88004350
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
080106 -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AEROBIC CONDITIONS
BACTERIA
BIOLOGICAL ADAPTATION
CELL CONSTITUENTS
CULTURE MEDIA
DESULFOVIBRIO
DNA
DOCUMENT TYPES
ENZYMES
HYDROGENASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLASMIDS
PROGRESS REPORT
RECOMBINANT DNA
SULFATE-REDUCING BACTERIA