Pyrethroid receptor in the insect Na sup + channel: Alteration of its properties in pyrethroid-resistant flies
- Centre National de la Recherche Scientifique, Nice (France)
Resistance to insecticides is a major problem in agriculture. ({sup 3}H)Saxitoxin binding experiments have shown that pyrethroid-sensitive and pyrethroid-resistant flies have the same amount of Na{sup +} channel protein in their brain membranes. Also, although flies are resistant to pyrethroids, they remain as sensitive to batrachotoxin, which is another type of Na{sup +} channel activators, as pyrethroid-sensitive flies. Pyrethroid binding sites have been characterized by use of the properties of pyrethroids to increase the specific ({sup 3}H)batrachotoxinin A 20{alpha}-benzoate binding component. K{sub 0.5} values for association of pyrethroids at the Na{sup +} channel of pyrethroid-sensitive flies are in the range of 0.15-0.25 {mu}M. Conversely, pyrethroids do not produce a significant increase of ({sup 3}H)batrachotoxinin A 20{alpha}-benzoate binding in pyrethroid-resistant flies even at high concentrations of the insecticide. It is concluded that linkage between pyrethroid and batrachotoxin binding sites is altered in the pyrethroid-resistant fly strains. This alteration is probably due to a drastically decreased affinity of the Na{sup +} channel for pyrethroids.
- OSTI ID:
- 5719168
- Journal Information:
- Biochemistry; (USA), Vol. 28:4; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INSECTICIDES
TOXICITY
RECEPTORS
BIOCHEMICAL REACTION KINETICS
BRAIN
CARBON 14 COMPOUNDS
CATIONS
CELL MEMBRANES
CROSS-LINKING
FLIES
SODIUM COMPOUNDS
TOXINS
TRITIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ANIMALS
ANTIGENS
ARTHROPODS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHARGED PARTICLES
CHEMICAL REACTIONS
DIPTERA
HYDROGEN COMPOUNDS
INSECTS
INVERTEBRATES
IONS
KINETICS
LABELLED COMPOUNDS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
POLYMERIZATION
PROTEINS
REACTION KINETICS
TOXIC MATERIALS
550201* - Biochemistry- Tracer Techniques