Zinc ions and alkaline pH alter the phosphorylation state of human erythrocyte membrane proteins
Since the phosphorylation state of the red cell membrane proteins in vitro is likely to be regulated by phosphorylation and dephosphorylation, this research was carried out to investigate the possible role of membrane-bound phosphatase activities. These studies were conducted with red blood cell ghosts and IOVs from normal individuals and from an individual with hereditary spherocytosis. In vitro phosphorylation with ({gamma}-{sup 32}P) ATP was conducted in the presence and the absence of Zn{sup ++}, or erythrocyte ghosts and IOVs were pretreated for 30 minutes at 37{degree}C and pH 7-11 in the presence and the absence of calf intestine alkaline phosphatase. The resulting phosphoproteins were analyzed by SDS-polyacrylamide gel electrophoresis, stained with Coomassie blue, and fluorographed. In the presence of Zn{sup ++}, the red blood ghosts, with or without pretreatment, demonstrated enhanced phosphorylation of membrane proteins, including band 4.2. Preincubation at pH 10 in the presence of absence of exogenous phosphatase further stimulates phosphorylation of these proteins. Under similar conditions, the erythrocyte membranes also demonstrated the ability to hydrolyze p-nitrophenyl phosphate and to remove {sup 32}P from red blood cell phosphoproteins.
- Research Organization:
- Hahnemann Univ. Hospital, Philadelphia, PA (USA)
- OSTI ID:
- 5712155
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
MEMBRANE PROTEINS
PHOSPHORYLATION
ZINC
BIOLOGICAL EFFECTS
ATP
CATIONS
CELL MEMBRANES
ELECTROPHORESIS
ERYTHROCYTES
MAN
PH VALUE
PHOSPHORUS 32
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHARGED PARTICLES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
ESTERASES
HYDROLASES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANES
METALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHATASES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques