Microbiology and physiology of anaerobic fermentations of cellulose
Technical Report
·
OSTI ID:5709984
The biochemistry and physiology of four major groups of anaerobic bacteria involved in the conversion of cellulose to methane or chemical feedstocks are examined. Aspects of metabolism which are relevant to the interactions and bioenergetics of consortia are being studied. Properties of the cellulolytic enzyme cluster of Clostridium thermocellum are investigated. Five different hydrogenases have been characterized in detail from anaerobic bacteria. Genes for different hydrogenases are being cloned and sequenced to determine their structural relationships. The role of metal clusters in activation of H{sub 2} is being investigated, as is the structure and role of metal clusters in formate metabolism. The function of formate in the total synthesis of acetate from CO{sub 2} and the role of this primary in anaerobes will be examined as well. Finally, these enzyme studies will be performed on thermophilic bacteria and new, pertinent species will be isolated. 50 refs., 3 figs., 1 tab.
- Research Organization:
- Georgia Univ., Athens, GA (United States). Research Foundation
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG09-86ER13614
- OSTI ID:
- 5709984
- Report Number(s):
- DOE/ER/13614-4; ON: DE91015239
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
550200 -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
ANAEROBIC DIGESTION
AZOTOBACTER
BACTERIA
BIOCHEMISTRY
BIOCONVERSION
BIOLOGICAL PATHWAYS
BIOMASS
BIOSYNTHESIS
BIOTECHNOLOGY
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CELLULOSE
CHEMICAL REACTIONS
CHEMISTRY
CLONING
CLOSTRIDIUM
DECOMPOSITION
DESULFOVIBRIO
DIGESTION
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
ENZYMES
FORMATES
HYBRIDIZATION
HYDROCARBONS
HYDROGENASES
HYDROLYSIS
LYSIS
MANAGEMENT
METHANE
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
OXIDOREDUCTASES
POLYSACCHARIDES
PROCESSING
PROGRESS REPORT
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
SULFATE-REDUCING BACTERIA
SYNTHESIS
TEMPERATURE DEPENDENCE
WASTE MANAGEMENT
WASTE PROCESSING
090900* -- Biomass Fuels-- Processing-- (1990-)
550200 -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
ANAEROBIC DIGESTION
AZOTOBACTER
BACTERIA
BIOCHEMISTRY
BIOCONVERSION
BIOLOGICAL PATHWAYS
BIOMASS
BIOSYNTHESIS
BIOTECHNOLOGY
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CELLULOSE
CHEMICAL REACTIONS
CHEMISTRY
CLONING
CLOSTRIDIUM
DECOMPOSITION
DESULFOVIBRIO
DIGESTION
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
ENZYMES
FORMATES
HYBRIDIZATION
HYDROCARBONS
HYDROGENASES
HYDROLYSIS
LYSIS
MANAGEMENT
METHANE
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
OXIDOREDUCTASES
POLYSACCHARIDES
PROCESSING
PROGRESS REPORT
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
SULFATE-REDUCING BACTERIA
SYNTHESIS
TEMPERATURE DEPENDENCE
WASTE MANAGEMENT
WASTE PROCESSING