Effect of platelet activating factor on endothelial permeability to plasma macromolecules
The effect of intrajugular administration of platelet activating factor (PAF-C16) on vascular permeability was examined in the guinea pig. To examine the loss of selective endothelial permeability, the extravasative effect of PAF was assessed by monitoring hemoconcentration and the plasma loss of /sup 125/I-albumin (6.7 nm), /sup 125/I-low density lipoproteins (22.0 nm) or /sup 125/I-very low density lipoproteins (62.1 nm). Extravasation was dose-dependent and began 1 min after PAF administration, continuing for 5-7 min. During extravasation, there was no evidence for selective plasma retention of any of the labeled plasma tracers, as measured by plasma radioactivity. These results suggest that PAF-induced extravasation is dose-dependent, with increases in vascular permeability sufficient to permit similar plasma efflux rates of albumin, low density lipoproteins and very low density lipoproteins.
- Research Organization:
- Sandoz Research Inst., East Hanover, NJ
- OSTI ID:
- 5708200
- Journal Information:
- Immunopharmacology; (United States), Vol. 8:3-4, Issue 3-4
- Country of Publication:
- United States
- Language:
- English
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551001* - Physiological Systems- Tracer Techniques