Field calibration of soil-core microcosms for evaluating fate and effects of genetically engineered microorganisms in terrestrial ecosystems

Technical Report · Mon Apr 01 04:00:00 EST 1991

DOI:https://doi.org/10.2172/5708022· OSTI ID:5708022

Bolton, H Jr; Fredrickson, J K; Bentjen, S A; Workman, D J; Li, S W; Thomas, J M

Pacific Northwest Laboratory compared intact soil-core microcosms and the field for ecosystem structural and functional properties after the introduction of a model genetically engineered microorganism (GEM). This project used two distinct microbial types as model GEMs, Gram-negative Pseudomonas sp. RC1, which was an aggressive root colonizer, and Gram-positive Streptomyces lividans TK24. The model GEMs were added to surface soil in separate studies, with RC1 studied throughout the growth of winter wheat (Triticum aestivum), while TK24 was studied throughout a ten month period. Also, RC1 was used in studies conducted during two consecutive field seasons (1988 to 1990) to determine how year-to-year field variability influenced the calibration of microcosms with the field. The main conclusions of this research were that intact soil-core microcosms can be useful to simulate the field for studies of microbial fate and effects on ecosystem structural and functional properties. In general, microcosms in the growth chamber, which simulated average field variations, were similar to the field for most parameters or differences could be attributed to the great extremes in temperature that occurred in the field compared to the microcosms. Better controls of environmental variables including temperature and moisture will be necessary to more closely simulate the field for future use of microcosms for risk assessment. 126 refs., 13 figs., 12 tabs.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• Sponsoring Organization: EPA; Environmental Protection Agency, Washington, DC (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5708022
• Report Number(s): PNL-7667; ON: DE91011258
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540210 -- Environment
Terrestrial-- Basic Studies-- (1990-)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANNUAL VARIATIONS
BACTERIA
BATTELLE PACIFIC NORTHWEST LABORATORIES
CALIBRATION
CEREALS
DATA
ECOSYSTEMS
EXPERIMENTAL DATA
GENETIC ENGINEERING
GRASS
GROWTH
INFORMATION
LILIOPSIDA
MAGNOLIOPHYTA
MICROCOSMS
MICROORGANISMS
NATIONAL ORGANIZATIONS
NUMERICAL DATA
PLANT GROWTH
PLANTS
PSEUDOMONAS
SIMULATION
SOILS
STREPTOMYCES
TERRESTRIAL ECOSYSTEMS
US DOE
US ERDA
US ORGANIZATIONS
VARIATIONS
WHEAT