Effects of chlorhexidine on the structure and permeability of hamster cheek pouch mucosa
This study examined the effects of chlorhexidine (CHD) on the clinical appearance, morphology, and in vitro permeability of hamster cheek pouch mucosa. The cheek pouches were treated daily for 3 weeks with topical applications of saline, 0.2% CHD, or 2.0% CHD. Treatment with 2.0% CHD resulted in the formation of discrete white lesions in every animal in the group, whereas no changes were identified in any animal treated with 0.2% CHD or saline. Upon microscopic examination it was determined that treatment with 2.0% CHD resulted in a statistically significant increase in epithelial thickness, when compared to the other groups, and the lesions were found to consist of hyperplastic areas of epithelium with associated inflammatory cell accumulations. Daily treatments with 2.0% CHD, 0.2% CHD or saline had no effect on the very low permeability of cheek pouch mucosa to /sup 14/C-CHD. However, treatment with 2.0% CHD resulted in decreased permeability to /sup 3/H/sub 2/O when compared to the other groups. Treatment with 2.0% CHD also resulted in a thickened permeability barrier, as determined using a tracer, horseradish peroxidase. It is concluded that topical applications of 0.2% T CHD have no detectable effect on cheek-pouch mucosa while applications of 2.0% CHD result in hyperplasia and a decrease in mucosal permeability. Results suggest that CHD should be used with caution clinically and at a concentration of 0.2% or less.
- Research Organization:
- Univ. of Iowa, Iowa City
- OSTI ID:
- 5706085
- Journal Information:
- J. Periodontol.; (United States), Vol. 55:10
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANTISEPTICS
TOXICITY
MUCOUS MEMBRANES
MORPHOLOGICAL CHANGES
PERMEABILITY
CARBON 14 COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
HAMSTERS
ORAL CAVITY
ANIMAL TISSUES
ANIMALS
BODY
DIGESTIVE SYSTEM
GERMICIDES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
RODENTS
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
551001 - Physiological Systems- Tracer Techniques