Hindered Rotational Diffusion and Rotational Jumps of Single Molecules

Journal Article · Sat Feb 28 23:00:00 EST 1998 · Physical Review Letters

DOI:https://doi.org/10.1103/PhysRevLett.80.2093· OSTI ID:570305

Ha, T; Glass, J; Enderle, T; Chemla, D S; Weiss, S ^[1]; Glass, J; Chemla, D S ^[2]

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1. Materials Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720 (United States)
2. Department of Physics, University of California at Berkeley, Berkeley, California 94720 (United States)

We extended the sensitivity of fluorescence polarization anisotropy measurements to the single molecule level by studying the polarization response of single fluorophores rapidly rotating in liquid. Comparison with a simple model calculation allows us to obtain information on the hindered motion as well as on the rotational diffusion in the presence of specific molecular interactions. We also observed rotational jumps between surface-bound and unbound states and show that they are, similar to jumps to long dark states, photoactivated. {copyright} {ital 1998} {ital The American Physical Society}

• Research Organization: Lawrence Berkeley National Laboratory
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 570305
• Journal Information: Physical Review Letters, Journal Name: Physical Review Letters Journal Issue: 10 Vol. 80; ISSN 0031-9007; ISSN PRLTAO
• Country of Publication: United States
• Language: English

Related Subjects

66 PHYSICS
ANISOTROPY
DIFFUSION
DNA
EXCITATION
FLUORESCENCE
MOLECULES
MOTION
POLARIZATION
ROTATIONAL STATES
SENSITIVITY