Dose-rate effects in plateau-phase cultures of S3 HeLa and V79 cells

Journal Article · Sat Sep 01 00:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575180· OSTI ID:5702990

Mitchell, J B; Bedford, J S; Bailey, S M

Dose-rate effects on cell survival were studied for log-, fed plateau-, and unfed plateau-phase cultures of V79 and S3 HeLa cells. For log-phase cultures, repair, cell-cycle redistribution, and cell division during exposure can contribute to the overall dose-rate effect, but their relative contributions are difficult to determine. With plateau-phase cultures, the cell-cycle times are greatly lengthened, for those cells that are in cycle. Hence, the contribution to the overall dose-rate effect of cell-cycle redistribution and cell division during the exposure could be minimized using plateau-phase cultures. With respect to the acute dose-survival curves, there was a clear loss in effectiveness when the dose rate was lowered to 154 rad/hr for both fed and unfed plateau-phase HeLa and V79 cells. There was no further reduction in effectiveness per unit dose, however, when the dose rate was reduced to 55 rad/hr. Since there was virtually no cell division or cell-cycle redistribution, it may be that a limit to the repair-dependent dose-rate effect at 37/sup 0/C has been reached at a dose rate of 154 rad/hr.

• Research Organization: Colorado State Univ., Fort Collins
• OSTI ID: 5702990
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 79:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CELL CYCLE
CELL DIVISION
DOSE RATES
DOSE-RESPONSE RELATIONSHIPS
HELA CELLS
IRRADIATION
LOW DOSE IRRADIATION
RECOVERY
REPAIR
SURVIVAL CURVES