Effects of radioprotectors on DNA polymerase I-directed repair synthesis and DNA strand breaks in toluene-treated and x-irradiated Escherichia coli

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576081· OSTI ID:5698049

Billen, D

In Escherichia coli made permeable to nucleotides by toluene treatment, a DNA polymerase I-directed repair synthesis is induced by exposure to x rays. This repair synthesis may be amplified and easily measured through inhibition of DNA ligase action. In an effort to learn more of the relationship between x-ray-induced strand breaks in cellular DNA and the extent of this repair synthesis, experiments designed to compare the influence of radioprotectors on both strand-break production and repair synthesis have been carried out. The results show that cysteamine, sodium formate, and glycerol not only protect against strand breaks but also reduce DNA polymerase I-directed repair synthesis. However, I/sup -/, an efficient hydroxyl radical scavenger, is not as effective a protective agent against strand breaks and does not measurably affect repair synthesis in our system.

• Research Organization: Univ. of Tennessee, Oak Ridge
• DOE Contract Number: AS05-76EV04568; W-7405-ENG-26
• OSTI ID: 5698049
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 95:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALI METAL COMPOUNDS
AMINES
AROMATICS
BACTERIA
CARBOXYLIC ACID SALTS
DNA
DNA REPLICATION
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
FORMATES
GLYCEROL
HYDROCARBONS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MEA
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATIONS
RADICALS
RADIOINDUCTION
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
SODIUM COMPOUNDS
STRAND BREAKS
THIOLS
TOLUENE
TRANSFERASES
X RADIATION