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U.S. Department of Energy
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Preconcentrating (within the broth) secreted extracellular proteins during a bakers' yeast fermentation

Conference · · Biotechnol. Bioeng. Symp.; (United States)
OSTI ID:5693703

Proteins secreted by yeast during the fermentation process are spacially fractionated (concentrated at a particular vertical position) within the fermentation vessel due to the phenomenon of bubble fractionation, despite moderately vigorous mixing. The degree of fractionation is influenced by the conditions in which the fermentation takes place. The broth pH strongly influences the extent of fractionation of specific proteins. In addition fractionation is enhanced under anaerobic conditions, presumably because there are more CO2 bubbles present for hydrophobic protein adsorption. The addition of moderate levels of salt to the broth reduces the fractionation for most (but not all) of the proteins.

Research Organization:
Venderbilt Univ., Nashiville, TN 37235, USA. Chemical Engineering Dept.
OSTI ID:
5693703
Report Number(s):
CONF-860508-
Journal Information:
Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 17; ISSN BIBSB
Country of Publication:
United States
Language:
English