Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1002/etc.5620120921· OSTI ID:5692428

Rattner, B A; Melancon, M J; Custer, T W; Hothem, R L; King, K A; LeCaptain, L J; Spann, J W ^[1]; Woodin, B R; Stegeman, J J ^[2]

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1. U.S. Fish and Wildlife Service, Laurel, MD (United States). Patuxent Wildlife Research Center
2. Woods Hole Oceanographic Inst., MA (United States)

Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins were measured in pipping black-crowned night heron (Nycticorax nycticorax) embryos collected from a reference site (next to the Chincoteague National Wildlife Refuge, VA) and three polluted sites (Cat Island, Green Bay, Lake Michigan, WI; Bair Island, San Francisco Bay, CA; West Marin Island, San Francisco Bay, CA). In a laboratory study, artificially incubated night heron embryos from the reference site were treated with 3-methylcholanthrene or phenobarbital. Compared to controls, 3-methylcholanthrene induced a greater than fivefold increase in activities of several monooxygenases and a greater than 100-fold increase in the concentration of immunodetected cytochrome P450 1A (CYP1A). Phenobarbital treatment resulted in only a slight increase in BROD activity but induced proteins recognized by antibodies to cytochrome P450 2B (CYP2B) by 2,000-fold. In a field study, activities of AHH, BROD, EROD, and ethoxycoumarin-O-dealkylase (ECOD) were up to 85-fold higher in pipping black-crowned night herons collected from Cat Island compared to other sites. Hepatic CYP1A and CYP2B cross-reactive proteins were detected in significantly more individuals from Cat Island than from the reference site. Greatest burdens of total PCBs and p,p[prime]-DDE were detected in embryos from Cat Island. Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins (AHH, BROD, EROD, ECOD, CYP1A, CYP1B) were significantly associated with total PCB burdens.

• OSTI ID: 5692428
• Journal Information: Environmental Toxicology and Chemistry; (United States), Journal Name: Environmental Toxicology and Chemistry; (United States) Vol. 12:9; ISSN ETOCDK; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BIOLOGICAL MARKERS
BIRDS
BODY BURDEN
CONDENSED AROMATICS
EMBRYOS
ENZYME INDUCTION
ENZYMES
GENE REGULATION
HYDROCARBONS
METABOLISM
MIXED-FUNCTION OXIDASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
SENSITIVITY
VERTEBRATES