Prostaglandin E and F2 alpha receptors in human myometrium during the menstrual cycle and in pregnancy and labor
The binding of prostaglandins E1 and F2 alpha has been studied in the human myometrium and cervix during the menstrual cycle and in the myometrium of pregnant patients at term before and during labor. Tritium-labeled prostaglandin E1 and F2 alpha binding was saturable and reversible. Scatchard analysis of tritium-labeled prostaglandin E1 binding was linear, which suggests a single class of high-affinity binding sites with an estimated apparent equilibrium dissociation constant of 2.5 to 5.4 nmol/L and inhibitor affinities of 0.9, 273, 273, and 217 nmol/L for prostaglandins E2, A1, B1, and F2 alpha, respectively. Scatchard analysis of tritium-labeled prostaglandin F2 alpha, binding was also linear, but the affinity of these binding sites was much lower, with an average dissociation constant of 50 nmol/L and inhibitor affinities of 1.6, 2.2, and 11.2 nmol/L for prostaglandins E1, E2, and A1, respectively. In nonpregnant patients, the concentrations and affinities of tritium-labeled prostaglandin E1 binding sites were similar in the myometrium during the proliferative and secretory phases of the menstrual cycle, but the concentration of these sites was much lower in the cervix. The concentration of the tritium-labeled prostaglandin E1 binding sites was significantly lower in the myometrium of pregnant patients at term than in the myometrium of nonpregnant patients. The concentrations and affinities of tritium-labeled prostaglandin E1 binding sites were not significantly different in the upper and lower myometrium of pregnant patients at term or in the myometrium of such patients before and during labor. The concentrations of the tritium-labeled prostaglandin F2 alpha binding sites during the menstrual cycle and in pregnancy at term were similar to those of tritium-labeled prostaglandin E1 binding sites.
- OSTI ID:
- 5692194
- Journal Information:
- Am. J. Obstet. Gynecol.; (United States), Vol. 8
- Country of Publication:
- United States
- Language:
- English
Similar Records
Quantitation of alpha 1-adrenergic receptors in porcine uterine and mesenteric arteries
( sup 3 H)rauwolscine binding to myometrial. alpha. sub 2 -adrenoceptors in pregnant guinea pig
Related Subjects
PROSTAGLANDINS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
AFFINITY
MENSTRUAL CYCLE
PATIENTS
PREGNANCY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
BODY
FEMALE GENITALS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques