ENDOR and ELDOR studies of x-irradiated polycrystalline dipeptides, myosin, and actomyosin

Journal Article · Thu Dec 27 04:00:00 EST 1979 · J. Phys. Chem.; (United States)

DOI:https://doi.org/10.1021/j100489a014· OSTI ID:5689379

Hwang, J S ^[1]; Dickinson, A C; Kispert, L D

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1. Univ. of Alabama, Tuscaloosa

ENDOR and ELDOR studies have been carried out for nine dipeptide powders as well as powders of myosin and actomyosin x ray irradiated at 77/sup 0/K in an attempt to characterize the final radical stable upon annealing between 183 and 260/sup 0/K. The dipeptides studied were glycylglycine, L-alanylglycine, glycyl-L-alanine, L-alanyl-L-alanine, glycyl-L-aspartic acid, glycyl-L-glutamic acid, glycyl-L-methionine, glycyl-L-serine, and L-lysyl-L-lysine. Nitrogen ENDOR spectra have been observed between 1 and 8 MHz for each powder and the nitrogen hyperfine and quadrupole tensor has been estimated. Analysis of the ENDOR, ELDOR, and ESR spectra indicates at least one of the final radicals in the dipeptide powders (except Gly-Gly, and possibly Gly-Glu, Gly-Ser) to be the decarboxylation product NH/sub 2/CHRCONHCHR' rather than just the abstraction type (NH/sub 3//sup +/-CHRONHCR'COO/sup -/) previously identified in irradiated dipeptide ices. A decarboxylation type radical is also present as a final radical in the irradiated myosin and actomyosin.

• OSTI ID: 5689379
• Journal Information: J. Phys. Chem.; (United States), Journal Name: J. Phys. Chem.; (United States) Vol. 83:26; ISSN JPCHA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400600* -- Radiation Chemistry
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DATA
DATA FORMS
ELDOR
ELECTROMAGNETIC RADIATION
ELECTRON SPIN RESONANCE
ENDOR
EXPERIMENTAL DATA
GLOBULINS
INFORMATION
IONIZING RADIATIONS
ISOLATED VALUES
MAGNETIC RESONANCE
MYOSIN
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RESONANCE
X RADIATION